Michael Cheika has handed debuts to Jordan Petaia and Jake Gordon, while Adam Ashley-Cooper will also start when Australia face Italy on Saturday.
Petaia, 18, will become the youngest Wallabies debutant since James O'Connor a decade ago when he starts on the left wing.
Scrum-half Gordon also gets his chance in Padova, with Cheika making six changes of personnel following the 9-6 defeat to Wales.
Ashley-Cooper will win his first cap for over two years on the right wing, so Israel Folau reverts to full-back.
Matt Toomua comes into the side at 10 with Bernard Foley moving to the centres alongside Samu Kerevi as Kurtley Beale drops to the bench.
Cheika has opted against resting captain Michael Hooper and David Pocock ahead of the showdown with England at Twickenham on November 24.
Full-back Jayden Hayward replaces Luca Sperandio in the only change to the Italy team following their 28-17 victory over Georgia.
Australia: Israel Folau, Adam Ashley-Cooper, Samu Kerevi, Bernard Foley, Jordan Petaia, Matt Toomua, Jake Gordon; Scott Sio, Folau Fainga'a, Taniela Tupou, Izack Rodda, Adam Coleman, Jack Dempsey, Michael Hooper (captain), David Pocock.
Replacements: Tatafu Polota-Nau, Jermaine Ainsley, Sekope Kepu, Rory Arnold, Pete Samu, Will Genia, Kurtley Beale, Dane Haylett-Petty.
Italy: Jayden Hayward, Tommaso Benvenuti, Michele Campagnaro, Tommaso Castello, Mattia Bellini, Tommaso Allan, Tito Tebaldi; Andrea Lovotti, Leonardo Ghiraldini (captain), Simone Ferrari, Alessandro Zanni, Dean Budd, Sebastian Negri, Jake Polledri, Braam Steyn.
|Holmes edges out Thomas to win fifth PGA Tour event|
|Hamlin wins crash-filled Daytona 500|
|Marotta: Inter have never doubted Icardi|
|Guardiola was questioned too – Zola says Sarri needs time|
|Icardi has no intention to leave Inter - Nara|
|Tuchel hails ´exceptional´ PSG mentality|
|Mbappe: PSG team better than my goals record|
|Opelka claims maiden ATP title in New York|
|Mbappe makes Ligue 1 history with wonderful volley|
|Saudi takeover of Manchester United dismissed|
|Stroman unhappy with Blue Jays´ lack of veteran leadership|
|Sanchez is far from finished at the top level, insists Solskjaer|
|Giggs: Give Man United job to Solskjaer|
|Robinson hails Morris´ ´special´ Roosters debut|
|Saint-Etienne 0 Paris Saint-Germain 1: Magical Mbappe snatches the points|
|Morris: I showed Roosters team-mates what I can do|
|Morris´ debut hat-trick guides Roosters to World Club Challenge glory|
|Icardi did not celebrate Inter win in dressing room, says Spalletti|
|Can: No privileges at Juventus, even for Ronaldo|
|Crusaders coach Robertson signs on for two more seasons|
|20-0 farce slammed as ´insult to sport´ by Italian football chief|
|Messi makes playing for Barcelona easier - Dembele|
|Alisson: Liverpool won´t feel Van Dijk loss against Bayern|
|SPAL coach Semplici left frustrated by VAR controversy|
|Jose Mourinho open to Ligue 1 job|
|Inter 2 Sampdoria 1: Nainggolan salvages win in Icardi´s absence|
|Muguruza and Kasatkina battle their way into Dubai second round|
|Cecchinato hammers home favourite Schwartzman to win Argentina Open|
|Toulouse resist Racing fightback to reclaim top spot|
|Icardi contract talks continue despite losing Inter captaincy|
|Halep ends Van Cleemput collaboration|
|Swansea City 4 Brentford 1: James-inspired turnaround books quarter-final berth|
|Doncaster Rovers 0 Crystal Palace 2: Eagles ease into FA Cup´s last eight|
|Windies star Gayle to retire from ODIs after World Cup|
|Wenger backs Henry to bounce back|
|20-0! Seven-man crisis club Pro Piacenza thrashed in Serie C farce|
|Monfils holds off Wawrinka for Rotterdam title|
|Walker hat-trick gets Rhinos up and running|
|Ramos red card an ´occupational hazard´, says Solari|
|Dan Carter to make Racing 92 return|
|Ashton ruled out for Wales clash|
|Nuno hails cup win as ´moment of joy´|
|Marcelo v Reguilon: Real Madrid mainstay losing left-back battle|
|Marcelo is an institution - Eusebio denies targeting Real Madrid left-back|
|I´d rather surround myself with people I really like - Osaka explains coach split|
|We do not have to kill Real Madrid forever - Odriozola|
|Bristol City 0 Wolves 1: Cavaleiro repeats cup heroics|
|Blackburn Rovers 0 Middlesbrough 1: Assombalonga strengthens Boro´s play-off credentials|
|Coman restricted to light training ahead of Liverpool clash|
|Solari refuses to concede LaLiga title race is over|
|Courtois: Real Madrid could not control Girona|
|Weary Kvitova reflects on ´crushing´ start to 2019|
|Umtiti in Barcelona squad to face former club Lyon|
|It´s hard to take - Maxwell laments Stars´ stunning Big Bash defeat|
|Pogba: Manchester United emulating ´Class of 92´|
|Real Madrid 1 Girona 2: Stuani and Portu seal stunning comeback|
|Boult, Mahmudullah fined by ICC|
|Neymar Senior: No chance of PSG star facing Manchester United|
|Broad expects sledging to subside in 2019 Ashes|
|Aguero targets historic quadruple|
|Scholes backs Solskjaer for Man United job|
|Finch hails ´special´ Renegades comeback|
|Sydney FC 1 Central Coast Mariners 1: Le Fondre strike averts shock defeat|
|Fox soars in Perth final to win World Super 6|
|Williams delay start to pre-season testing|
|Renegades crowned BBL champions after stunning Stars collapse|
|What we learned from NBA All-Star Saturday|
|Kaepernick´s attorney tips Patriots to make move for QB|
|Tebow: I told Murray to follow his heart|
|Mourinho created unhealthy atmosphere – Sanchez|